Tag Archives: breast cancer

Cancer cells exchange leaders during invasion (PNAS)

By Catherine Zandonella, Office of the Dean for Research

A new study has found that cancer cells appear to exchange leading roles as they migrate out of a tumor in the early stages of invasion, or metastasis, of other sites in the body. Metastatic cancer accounts for more than 90% of cancer-related deaths.

A team led by Robert Austin, professor of physics at Princeton University, found that individual cancer cells take turns as trailblazers when they carve their way through the dense wall — known as the extracellular matrix — that stands between a tumor and the blood vessels which can carry the cells to other parts of the body.

The researchers also found that the cells leave the tumor in search of food, since cells that had plenty of available nutrients did not migrate. The finding reinforces the hypothesis that metastasis occurs when tumors become so densely packed that blood vessels cannot penetrate the interior and cancer cells must migrate to survive.

The researchers included first author Liyu Liu of the Chinese Academy of Sciences; Guillaume Duclos of the National Center for Scientific Research in Paris; Bo Sun, Jeongseog Lee, Amy Wu, Howard Stone and James Sturm of Princeton University; Yoonseok Kam and Robert Gatenby of H. Lee Moffitt Cancer Center in Tampa; and Eduardo Sontag of Rutgers University. The article appeared in the Proceedings of the National Academy of Sciences.

To study cancer cell behavior, the researchers constructed a small chamber with three compartments arranged like floors in an apartment building. On the bottom floor was a well of glucose, the preferred food for metastatic cells. The middle floor contained a dense layer of collagen, a protein that makes up the extracellular matrix that surrounds tumors. On the top floor they placed metastatic cancer cells, which were labeled with fluorescent dye for visibility. They trained a microscope and camera on the chamber.

Through the microscope, the researchers filmed the cancer cells as they moved down through the chamber toward the glucose. The researchers found that a single cell would become the leader for some time, then drop back as another cell took the lead in what the authors term a “collective invasion strategy.” They also found that the collagen was pushed aside, leaving a wake in which cells behind the leader could travel.

Because the collagen is very dense, the cells must expend a lot of energy to reach the glucose, and indeed the researchers found that cells without a need for glucose did not bother to burrow down into the collagen. The researchers used collagen with a density similar to that of human breast tissue.

The study adds to the growing understanding of metastasis and could serve to assist researchers in developing strategies for its prevention.

Liyu Liu, Guillaume Duclos, Bo Sun, Jeongseog Lee, Amy Wu, Yoonseok Kam, Eduardo D. Sontag, Howard A. Stone, James C. Sturm, Robert A. Gatenby, and Robert H. Austin. Minimization of thermodynamic costs in cancer cell invasion. PNAS January 14, 2013 201221147.

Read the paper (open access).

This work was supported by the National Science Foundation and the National Cancer Institute.

The role of breast structure in tumor development (PNAS)

Why do some breast tumors grow aggressively while others grow slowly? In this study, researchers found that the stiffness of the cells in the area around an emerging tumor influences its ability to grow and invade the breast. Using a 3-D fabrication process, the researchers created artificial breast ducts containing normal breast cells and a single tumor cell. They found that regions characterized by stiffness among the normal cells were more likely to give rise to tumors that are aggressive and invasive, while regions that were less stiff gave rise to tumors that are less invasive.

Eline Boghaert, Jason P. Gleghorn, KangAe Lee, Nikolce Gjorevski, Derek C. Radisky, and Celeste M. Nelson. Host epithelial geometry regulates breast cancer
cell invasiveness. Published online before print November 12, 2012, doi: 10.1073/pnas.1118872109 PNAS November 12, 2012

Read the abstract.